| Serum and glucocorticoid inducible kinase SGK phosphorylates and negatively regulates B Raf . ^^^ Here we investigated the regulation of B Raf by serum and glucocorticoid inducible kinase , SGK , which shares close sequence identity with the catalytic domain of Akt but lacks the pleckstrin homology domain . ^^^ We observed that SGK inhibits B Raf activity . ^^^ A comparison of substrate specificity between SGK and Akt indicates that SGK is a potent negative regulator of B Raf . ^^^ In contrast to Akt , SGK negatively regulates B Raf kinase activity by phosphorylating only a single Akt consensus site , Ser ( 364 ) . ^^^ |