| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.8394475 |
| Here we show that PDK 1 activates SGK in vitro by phosphorylating Thr 256 . 0.8394475^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| Functional counterparts of mammalian protein kinases PDK 1 and SGK in budding yeast . ^^^ Activated PDK 1 then phosphorylates and activates downstream protein kinases , including protein kinase B ( PKB ) / c Akt , p 70 S6 kinase , PKC isoforms , and serum and glucocorticoid inducible kinase ( SGK ) , thereby eliciting physiological responses . ^^^ Purified Pkh 1 activated mammalian SGK and PKBalpha in vitro by phosphorylating the same residue as PDK 1 . ^^^ CONCLUSIONS : Pkh 1 and Pkh 2 function similarly to PDK 1 , and Ypk 1 and Ykr 2 to SGK . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| Cotransfection of PDK 1 with SGK caused a 6 fold activation of SGK activity , whereas kinase dead PDK 1 caused no activation . ^^^ GST pulldown assays revealed a direct interaction between PDK 1 and the catalytic domain of SGK . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| The Ypk 1 protein was reported to be a functional homologue of the mammalian protein kinase SGK , which is a downstream kinase of 3 phosphoinositide dependent kinase 1 ( PDK 1 ) . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| Both H ( 2 ) O ( 2 ) and vanadate treatments increased the activity of PDK 1 when the serum / glucocorticoid regulated kinase ( SGK ) was used as substrate . ^^^ Tyrosine phosphorylation of PDK 1 by the Abl tyrosine kinase also increased the activity of PDK 1 toward SGK and Akt . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| The PIF binding pocket in PDK 1 is essential for activation of S6K and SGK , but not PKB . ^^^ PDK 1 activates S6K , SGK and PKB isoforms by phosphorylating these kinases at their T loop . ^^^ We demonstrate that a pocket in the kinase domain of PDK 1 , termed the ' PIF binding pocket ' , plays a key role in mediating the interaction and phosphorylation of S6K1 and SGK 1 at their T loop motif by PDK 1 . ^^^ Our data indicate that prior phosphorylation of S6K1 and SGK 1 at their hydrophobic motif promotes their interaction with the PIF binding pocket of PDK 1 and their T loop phosphorylation . ^^^ Thus , the hydrophobic motif phosphorylation of S6K and SGK converts them into substrates that can be activated by PDK 1 . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| Like PKB , SGK 1 is activated by phosphorylation in response to signals that stimulate phosphatidylinositol 3 kinase , and this is mediated by 3 phosphoinositide dependent protein kinase 1 ( PDK 1 ) and another protein kinase that has yet to be identified . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| PKB / Akt , S6K , SGK and RSK are mediators of responses triggered by insulin and growth factors and are activated following phosphorylation by 3 phosphoinositide dependent protein kinase 1 ( PDK 1 ) . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| This effort has led to nanomolar to micromolar inhibitors of 11 new targets including p90RSK , KDR , c Kit , Fes , MAPK 1 , PAK 2 , PDK 1 , PKCtheta , PKD 2 , Rsk 1 , and SGK . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| Activation of receptor tyrosine kinase pathways or the phosphoinositide dependent kinase 1 ( PDK 1 ) lead to SGK dependent CREB phosphorylation . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| The catalytic domain of serum and glucocorticoid induced protein kinase ( SGK ) is 54 % identical with protein kinase B ( PKB ) and , like PKB , is activated in vitro by 3 phosphoinositide dependent protein kinase 1 ( PDK 1 ) and in vivo in response to signals that activate phosphatidylinositol ( PI ) 3 kinase . ^^^ SGK 2 and SGK 3 are activated in vitro by PDK 1 , albeit more slowly than SGK 1 , and their activation is accompanied by the phosphorylation of Thr ( 193 ) and Thr ( 253 ) respectively , the residues equivalent to the Thr in the ' activation loop ' of PKB that is targeted by PDK 1 . ^^^ The PDK 1 catalysed phosphorylation and activation of SGK 2 and SGK 3 , like SGK 1 , is greatly potentiated by mutating Ser ( 356 ) and Ser ( 419 ) respectively to Asp , these residues being equivalent to the C terminal phosphorylation site of PKB . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| IGF 1 induced a similar phosphorylation of CRHSP 24 in ES ( embryonic stem ) cells from wild type mice or mice that express the PDK 1 ( 3 phosphoinositide dependent kinase 1 ) mutant ( PDK 1 [ L155E ] ) that activates PKBalpha normally , but can not activate SGK . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| Kinase assays and the phosphorylation of AKT , GSK 3alpha / beta , PDK 1 , ERK1 / 2 , SGK , p 38 , FAK , EGFR , JAK 2 , PKC isoforms , and the cleavage of poly ( ADP ribose ) polymerase ( PARP ) were examined in treated and untreated cell lines . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| The disruption of the hydrophobic groove ablates activation of S6K , RSK and SGK , but the role of the phosphate groove in regulating the function of PDK 1 has not been explored in vivo . ^^^ However , in contrast to S6K , RSK and SGK , hydrophobic motif phosphorylation of these enzymes is not thought to control their activation by PDK 1 . ^^^ These findings provide genetic evidence that the phosphate groove of PDK 1 is required for maximal activation of isoforms of S6K , SGK and RSK , but not PKC . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| Similar to its isoforms SGK 2 and SGK 3 , SGK 1 is activated by insulin and growth factors via phosphatidylinositol 3 kinase and the 3 phosphoinositide dependent kinase PDK 1 . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| IGF 1 up regulates K+ channels via PI 3 kinase , PDK 1 and SGK 1 . ^^^ In conclusion , IGF 1 through PI 3 kinase , PDK 1 and SGK 1 up regulates Kv channels , an effect required for the proliferative action of the growth factor . . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| The SGK 1 protein belongs to the AGC gene family of kinases that are regulated by phosphorylation mediated by PDK 1 . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| Using pull down assays and co transfection experiments , we demonstrated that the DSFL tail of SGK 1 interacts with the first PDZ domain of NHERF 2 and the PIF of NHERF 2 binds to the PIF binding pocket of PDK 1 to form an SGK 1 NHERF2 PDK 1 complex . ^^^ Formation of the protein complex promoted the phosphorylation and activation of SGK 1 by PDK 1 . ^^^ Thus , it was suggested that NHERF 2 mediates the activation and phosphorylation of SGK 1 by PDK 1 through its first PDZ domain and PIF motif , as a novel SGK 1 activation mechanism . . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| In contrast , IGF 1 still induced normal phosphorylation of WNK 1 in PDK 1 ( L155E / L155E ) knock in ES cells in which PKB , but not S6K ( p 70 ribosomal S 6 kinase ) or SGK 1 ( serum and glucocorticoid induced protein kinase 1 ) , is activated . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| The signaling involved in activation of voltage gated K ( + ) channel Kv1 . 3 includes the phosphatidylinositol 3 ( PI 3 ) protein kinase , 3 phosphoinositide dependent protein kinase PDK 1 and the serum and glucocorticoid inducible kinase SGK 1 . ^^^ |
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| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| Akt inhibitory phenoxazines did not inhibit the activity of recombinant phosphatidylinositol 3 ' kinase , PDK 1 , or SGK 1 but potently inhibited the kinase activity of recombinant Akt and Akt deltaPH , a mutant lacking the pleckstrin homology domain . ^^^ |
|
| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O00141 and O15530 |
Pubmed |
SVM Score :0.0 |
| NA |
|